Which one has the least amount of bacteria growing near it? Take one of the sterile swabs and wipe it over the teeth and around within the mouth. Mouthwash Experiment V ariables, Questions and Hypotheses After collecting the oral samples, there are several questions that the students should answer to ensure that they understand the scientific method of this experiment. Bacteria move using flagella, which are tiny structures that beat in a circular motion. The effects of mouthrinses could be due to thymol and eucalyptol, the volatile oils that have proven activity against bacteria. Coli to cover the dish. Topical antiseptics and antibiotics in the initial therapy of chronic adult periodontitis: microbiological aspects. They can also sense food or toxins.
What are these are the dependent variables. Take inoculating loop, and streak plate for isolation. They control it in two ways: by killing the bacteria cells, and by stopping the bacteria from reproducing. Take notes of your observations and make drawings. Remove the cover of one of the petri dishes and begin to rub the swab across the agar in a zigzag pattern.
The experiment's purpose was to show the effect of commercial mouthwashes, containing varying amounts of alcohol, on oral bacteria growth. Experimental Variables What Is Being Manipulated? It attaches the tooth to the jawbone. The source of the bacteria. Mouthwash ingredients Mouthwash is a product used to enhance oral hygiene and uses fluoride to protect against tooth decay. Once the sample is obtained, wipe the swab over the corresponding section of agar, using the same application pattern as for Section 1. You can also test different formulations of the same brand of mouthwash. The mean, variance, and t-test proved the hypothesis to be correct.
For replication each group we used was made up of three petri-dishes containing 5 filter paper dots that had been dipped in the pre-determined mouthwash. A study evaluated the antimicrobial effect of some topical oral agents including Chlorhexidine 0. Mouthwashes can inhibit dental plaque, and are widely used to maintain oral hygiene. More accurate tools could have been used to conduct the experiment; an incubator would have controlled the environment of the Petri dishes better than a heat lamp, as it would be easy to set to a certain temperature and humidity. An in vitro antimicrobial comparison of miswak extract with commercially available non-alcohol mouthrinses.
Repeat the swabbing and applying to petri dish with a new cotton swab for the remaining 5 petri dishes. The range, as calculated from the data table, The Effect of Commercial Mouthwash Brands on Oral Bacteria, for Crest Pro-Health was 9. Students with last names starting with N — Z, are to use mouthwash containing alcohol Listerine. Crest Pro-Health and Act Total Care killed 12. Once the bacteria break it down, our intestines are able to absorb it, giving us more nutrients from our food. The growth of both E. Although alcohol is commonly regarded as an effective bacteria killer, it also dries out the mouth, which can make the problem worse.
The temperature should be around 95-120 degrees to kill some of the harmful bacteria. What kind of soap works best for cutting down on the bacteria on our hands? The protocol of any experiment is to limit the number of variables involved, making analysis easier after you collect the data. Fluoride also helps to fight cavities. . Add one square of plain blotter paper to test if blotter paper by itself has any effect.
For the experiments, volunteers brushed a minimum of twice daily with a manual toothbrush. Neither you, nor the coeditors you shared it with will be able to recover it again. I based my hypothesis off of the fact that antiseptic mouthwashes are well known for their bacteria killing properties. You have chosen a very interesting science project and you will learn a lot about the very fascinating world of microbiology. Determination of the in situ bactericidal activity of an essential oil mouthrinse using a vital stain method.
It should keep for a couple of weeks. As interesting as this study turned out to be, there were no other studies that we were able to find to compare our results to! A study of the bacteria associated with advancing periodontitis in man. You also need a source for bacteria, and this is not hard to find! Seal the edges of your agar plate with tape. Chris Try the links in the for more information on. Effect of an antiseptic mouthrinse on salivary microbiota.
There are really two questions being investigated in this experiment; two independent variables that are manipulated. As spores, the bacteria can survive for hundreds, even thousands of years. Later, 200ml of uninoculated medium was included as a sterility control blank. Place the petri dishes in a stable environment and allow them to set or place them in a refrigerator over night. Even bacteria that lives safely on our skin can make us sick if it gets inside our bodies through our mouths or cuts and scrapes.
With this method, you will need to occasionally refill the cooler with hot water, so that the temperature of the yogurt stays consistent. Insert in colorimeter and measure the density. Leave the culture dishes exposed for about an hour. These kinds of bacteria are called pathogens. ~ Experimental Variables ~ What Is Being Manipulated? However, they can kill good bacteria along with the bad.