Capillary flow The first 2-3 drops of blood should be wiped away using a clean gauze pad. From farmers to pharmacists, copper sulphate is a chemical salt with interesting and differentiated properties. The sensitivity, specificity, predictive values and correlation between the reference method and HemoCue were assessed. To resolve the problem, the other method of measuring specific gravity using refractometer was entertained, but was not employed as the method requires removal of drops of copper sulphate solution for the tests, decreasing the volume of the solution. New noninvasive methods are now available for rapid, noninvasive predonation haemoglobin screening. It corrects copper deficiencies in the soil and animals and stimulates farm animals' growth.
United State Environment Protection Agency. Copper sulphate also inhibits bacterial growth such as Escherichia coli. The copper sulphate CuSo 4 method used to screen for blood abnormalities can give inaccurate results if strict quality control is not applied. Cyanmethaemoglobin method is based on the conversion of haemoglobin into cyanmethaemoglobin. Hemoglobin determined by the HemoCue method is comparable to that determined by the other methods. This study examined the agreement between a portable hemoglobin photometer and a laboratory analyzer in determining hemoglobin level in pregnant women.
Iron deficiency is the most common and widespread nutritional disorder in the world and the only nutrient deficiency with significant prevalence also in industrialized countries. In Canada 2% of all blood donors, or approximately 25,000 individuals annually attempt to make a blood donation but are deferred because their Hb level does not meet these minimum accepted standards. Consent forms have been secured for the publication of information about patients or cases; otherwise, authors have declared that all means have been exhausted for securing consent. Please see appendix B and C for the preparation of the copper sulphate solution. According to the Indian Drugs and Cosmetics Act, 1940 for blood donation, the minimum acceptable hemoglobin Hb is 12.
However, the visual comparison is susceptible to inter-observer variability and a low sensitivity has been found in some studies. The free hemoglobin is oxidized to methemoglobin which is then converted into azide methemoglobin, a stable colored complex. Method of analysis and sampling site need to be taken into consideration in field studies. In 100 of the donors, earstick hemoglobin levels were also measured on the HemoCue system. Blood samples in the group were rotated to complete the 50 tests.
The blood is dropped 1 cm above the surface of the copper sulphate solution. A measurement takes between 15 - 60 seconds, depending on the hemoglobin concentration. The measured specific gravity at the start of experiment, the specific gravity after the first twenty-five tests and every additional five tests to complete fifty tests were tabulated per experiment. When water is then added to the anhydrous compound, it turns back into the pentahydrate form, regaining its blue color, and is known as blue vitriol. At the same time, the recovery of inappropriately deferred donors by HemoCue could indicate its usefulness as a secondary screening method.
Full blood counts were done on each sample using the Coulter Model T890. In contrast, an Hb standard of 125 g per L in the copper sulfate test could achieve a more optimal sensitivity of 79 percent and specificity of 78 percent. Thus, a 100-mL copper sulphate solution can be used for fifty tests using samples of various hemoglobin levels at room temperature and at a higher temperature. The HemoCue® hemoglobin photometer has been used for these purposes. The patient must not wear a ring on the finger as this may obstruct the blood circulation.
Tondon R, Verma A, Pandey P, and Chaudhary R. Specimen Type: Blood Description: Copper Blood Test This test measures the amount of copper in the blood. A dilute solution of copper sulfate is used to treat fishes for parasitic infections, and is also used to remove snails from aquariums. The hemoglobin concentration is read directly from the calibration tube. Because copper sulphate exothermically dissolves in water, it gives off heat and can substantially raise the temperature of the water in which it is added. Instead, it controls invasive aquatic plants growing near public water or sewer pipes. Hence when a drop of blood is placed in a solution having specific gravity of 1.
Anhydrous Copper sulfate is 39. This subset of the donor population shows wide variation in Hb level over time, and this is largely independent of iron supplementation or inter-donation interval. Capillary sampling technique Both accuracy and reliability of hemoglobin measurements can be affected by pre-analytical errors. It often highlights the green tints of the specific dyes. The Hb levels of 131 32. Out of these, 65 donors had Hb values between 12.
Experiments 1, 2 and 3 made use of samples from groups A, B and C, respectively and were conducted at room temperature. The method is simple and cheap but rather inaccurate. As a non-agricultural , is it used to control invasive and the roots of plants situated near water pipes. This is one of the limitations of Copper Sulphate method in hemoglobin screening. Since no study was found stating the difference between the weight of anticoagulated blood and blood without anticoagulant, it was assumed that the anticoagulant present in the blood collection tubes could affect the result of the experiment by diluting the blood.