The volume of the solution was controlled by using the same amount of solution for the test tubes. In this lab, you will study an enzyme that is found in the cells of many living tissues. Substrate concentration, temperature, and pH all affect the chemical reaction. This is because enzymes begin to denature and not work as properly when they are placed outside their optimal temperature range. Enzymes are specific; different enzymes only react with certain substrates which results in the lock and key model, where the shapes enzymes and substrates match with their corresponding half. Most people are born producing it, but often make less of it as they age, which causes lactose intolerance the symptoms for this include nausea, bloating, and diarrhea to name a few. Measure the gas levels in the graduated cylinder at 30-second intervals for 10 minutes.
Push it into the hydrogen peroxide with a stirring rod. Some enzymes… 1946 Words 8 Pages The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Allow reaction vessel to stabilize in water bath for 2-3minutes before making the run. The procedure includes using pH5, H202, Enzyme Extract, and Guaiacol and calibrating a spectrophotometer to determine the effect of enzyme concentration. This is because the heat degraded the catalase enzyme, making it incapable of processing the hydrogen peroxide. Subsequently, in this experiment, hydrogen peroxide will be mixed with various levels of pH buffers, which then will be broken down by catalase to test how effectively our cells remove peroxide in our body.
Obtain a small amount of catalase solution in a 50 mL beaker. EvaluationEven though the experiment was performed carefully to minimize errors, there were still errors which may have changed the results. VariablesIndependent variable: the amount of catalase and peroxideDependent variable: the pressure of the oxygen after the catalase has reacted with the peroxideControlled variables: The size of the tubes were controlled by using the same sized test tubes for the experiment. Equipment: v Amylase solution 0. As hydrogen peroxide enters the active site of the catalase enzyme, it interacts with two amino acids, causing a proton to transfer between the oxygen atoms.
This temperature is about 91. Enzymes are most often proteins and theirthree-dimensional shape is important to their catalytic activity. It might seem strange to use dead cells to study the function of enzymes. We then found out our hypothesis is accurate because it proved that 664 Words 3 Pages Lab Report on Measuring the Rate of Conversion of Hydrogen Peroxide using Enzyme Catalysis In essence, the main objective was to use chemical titration to measure and then calculate the rate of conversion of hydrogen peroxide H2O2 to water and oxygen by using the enzyme catalase. We measured the height of each bubble column in each test tube with a ruler. A water bath that hasbeen chilled with ice to 5C. The apple sample is simply carbohydrates meant as food for the apple seed and will have no enzymes in it, thus no reaction was observed.
For example, to get energy out of amolecule of glucose, a series of reactions must take place in aspecific order to break the bonds between the carbons of the glucosemolecule. You are observing catalase breaking hydrogen peroxide into oxygen and water. Materials andMethods: At your lab table, youwill find everything you need to conduct today's experiments. Introduction This lab covered the measuring of the effects of changes in temperature, pH, and enzyme concentration on reaction rates of an enzyme catalyzed reaction in a controlled experiment. The 0 M copper sulfate data point is the valuegenerated in Part A 1: three disks soaked in catalase. Also, the equipment used to calculate the rate of gas production could possibly be calibrated before the experiment to ensure there are no problems with the equipment.
We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In this catalase and hydrogen peroxide experiment, we will discover how enzymes act as catalysts by causing chemical reactions to occur more quickly within living things. Enzymeactivity is influenced by many factors. You will be using chicken or beef liver. The enzyme that will be tested in this experiment is catalase, which is essential in breaking down hydrogen peroxide, another important part of this investigation.
Put one test tube of liver and one of H 2O 2 into an ice bath. The rates do increase in the beginning, but continues to rise after the pH of 9. Purpose: The purpose of this lab was to study the r ates of enzyme-catalyzed reactions through rate of appearance of product, disappearance of substrate, and pr essure of the product as it appears. We let them sit for 15 minis. Record the levels in your data tables Data Tables Graphs Conclusion In conclusion, the results supported the hypothesis that the amount of oxygen released decreased due the the temperatures being outside of the optimal range of the enzyme, yeast. Part B - What Tissues Contain Catalase? Increase in temperature causes molecules to move faster. The room temperature potato produced the most bubbles because catalase works best at a room temperature.
After dropping the Hydrogen Peroxide on the apple samples there was no reaction indicated therefore receiving a rating of 0. Protocol Work as a team of 4 at your table to perform theexperiment. How do living cells interact with the environment around them? After testing liver, apple, and potato it was concluded that liver contained the most catalase. Chemical reactions involve the breaking and reforming ofchemical bonds between molecules substrate s of the reaction ,which are transformed into different molecules product s of thereaction. The enzyme studied in this lab was catalase. The final test focused on the reaction rate of liver in varying pH solutions.
Another limitation could possibly be the use of technology, loggerpro more specifically, because it may not be accurate because of not calibrating the equipment. Remove the test tube from the hot water bath, allow it to air cool, then pour out the water. Wootton on October, 8 2015. Structure and Molecular Mechanism The catalase enzyme is comprised of four polypeptide chains, with each chain containing more than 500 amino acids. At your table you will find a small rectangularbottle fitted with a rubber stopper and metal tube reaction vessel ,a 100 ml graduated cylinder and holder, a plastic pan, and a supplyof small pieces of filter paper. Put a piece of liver into the bottom of a clean test tube and cover it with a small amount of water. In fact, your cells are always making poisonous chemicals.
If you had to rely onthese reactions to take place spontaneously, you would wait a verylong time -- you wouldn't be here! Synthesis: How does pH affect the activity of enzymes? The liver catalase was then tested and resulted in the largest reaction that was given a rating of 5 due to the speed and magnitude. Remove the disks, then thoroughly rinse anddry your reaction vessel A. Through the testing the enzyme at different temperatures, and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Place 2 ml of hydrogen peroxide in each of 3 clean test tubes and then add each of the three test substances to the tubes. Conclusion Through our experimentation the reaction between the enzyme catalase and hydrogen peroxide was tested. Results When the substrate of hydrogen peroxide was added to the substances each performed a vastly different reaction.